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FAQ DURAN® TILT

a) DURAN® TILT Glass Bottle FAQs

1.Are the TILT bottles made from the same glass as the DURAN® original GL 45 bottles?
Yes, the glass is identical. The TILT bottles are made from DURAN® borosilicate 3.3 glass, and have identical performance characteristics, in terms of chemical and thermal resistance as the DURAN® original GL 45 bottles.
2.Do the TILT bottles have the same volume of head space as the DURAN® original GL 45 bottles?
Additional headspace (above the nominal volume) is important in case of foaming, and for the effective mixing of additions such as serum. The TILT bottles have a similar headspace to the DURAN original GL 45 bottles.
3.Are the TILT bottles and GL 56 caps “clean” when we receive them or do you recommend additional cleaning before use?
No, neither the TILT bottles nor the caps are guaranteed to be clean. We do not take any special precautions after manufacture to ensure cleanliness. We always recommend that our customers clean the TILT bottles and caps in a validated washing system before use. For further information about cleaning labware, download our free guide (available in English, German or French) at: www.aklab.de
4.How should the TILT bottles and GL 56 caps be depyrogenised?

The efficiency of the depyrogenisation process is directly related to the cleanliness of the laboratoryware prior to treatment. Therefore the equipment must be thoroughly cleaned especially prior to the depyrogenisation using the chemical hydrolysis / inactivation method.

  1. Dry heat depyrogenisation recommendations
    (For the TILT glass bottle only - not applicable to the GL 56 screw cap)
    • +200 °C for 60 minutes (dry heat)

  2. Depyrogenisation recommendations for the GL 56 screw cap
    The DURAN® GL 56 screw cap is moulded from polypropylene and must not be exposed to temperatures higher than +140 °C.
    One method is dilution or rinsing. The amount of endotoxin is washed away or reduced using WFI (Water for injection USP<1231>). However aliphatic polymers such as polypropylene have a specific affinity (due to ionic and hydrophobic interactions) for binding endotoxins. We recommend that a chemical hydrolysis / inactivation or oxidation method is used for the GL 56 screw cap.

    Hydrolysis method using an acid or base:
    • 0.05M HCl at 100 °C for 30 minutes.
    • 1% glacial acetic acid 2 -3 hours at 100 °C
    • Or 0.5 M NaOH at 50 °C for 30 minutes.

    Oxidation method
    • 5% hydrogen peroxide
    • Peracetic acid (PA) at 18°C to 30°C

5.How should the TILT bottles and GL 56 caps be sterilised?

  1. Steam sterilization recommendations
    (For the TILT glass bottle and the GL 56 screw cap)
    • +121 °C for 20 minutes (saturated steam at ca. 250 kPa)
    • +134 °C for 5 minutes (saturated steam at ca. 300 kPa)
    • During steam sterilization, the GL 56 screw cap should only be loosely applied (maximum one turn - do not tighten).

  2. Dry heat sterilization recommendations
    (For the TILT glass bottle only - not applicable to the GL 56 screw cap)
    • +160 °C for 180 minutes (dry heat)
    • +170 °C for 60 minutes (dry heat)
    • +180 °C for 30 minutes (dry heat)

6.Can the TILT glass bottle be safely used for sterile filtration?
The TILT bottle is designed to be vacuum resistant. The maximum recommended vacuum is -700 mbar / 70 kPa / 20.7 inch of mercury Recommended Safe Working Practices Always wear eye protection when using the TILT bottle under vacuum. As with all laboratory glassware operated under vacuum, we recommend the use of a suitable safety screen. If the TILT bottle is accidently dropped, scratched or damaged in any way, we strongly recommend that it should not be used, and be replaced.

b) DURAN® TILT GL 56 Screw Cap FAQs

1. How do I determine the Retrace code on the white GL 56 cap?
The Retrace code is located on the inside of the GL 56 cap. There is a moulded “clock” showing a letter (A-Z) and numbers (1 - 12). The Retrace code is either a 2- or 3-character code in this format: Letter + Number - A1, A2…A12, B1, B2….B12 etc.
2. What material are the white coloured TILT GL 56 caps made from?
The white coloured TILT GL 56 caps are moulded from a medical grade, non-cytotoxic, virgin polypropylene homopolymer.
3. What does the TILT GL 56 cap have five small feet around its base?
When the screw cap is placed facedown inside a cell culture hood, the small feet raise the cap slightly off the work surface to reduce the risk of contamination entering the inside the cap.
4. How should the GL 56 caps be depyrogenised?

The efficiency of the depyrogenisation process is directly related to the cleanliness of the laboratoryware prior to treatment. Therefore the equipment must be thoroughly cleaned especially prior to the depyrogenisation using the chemical hydrolysis / inactivation method.

The DURAN® GL 56 screw cap is moulded from polypropylene and must not be exposed to temperatures higher than +140 °C.
One method is dilution or rinsing. The amount of endotoxin is washed away or reduced using WFI (Water for injection USP<1231>). However aliphatic polymers such as polypropylene have a specific affinity (due to ionic and hydrophobic interactions) for binding endotoxins. We recommend that a chemical hydrolysis / inactivation or oxidation method is used for the GL 56 screw cap.

Hydrolysis method using an acid or base:
• 0.05M HCl at 100 °C for 30 minutes.
• 1% glacial acetic acid 2 -3 hours at 100 °C
• Or 0.5 M NaOH at 50 °C for 30 minutes.

Oxidation method
• 5% hydrogen peroxide
• Peracetic acid (PA) at 18°C to 30°C

c) DURAN® TILT Protective Light Shield FAQs

1. What material is the DURAN® TILT Protective Light Shield manufactured from?
The DURAN® TILT Light Shield is moulded from a food grade silicone rubber. The silicone contains an ultraviolet (UV) light blocking pigment.
2. What is the function of the DURAN® TILT Protective Light Shield

The Light Shield has a number of protective functions:
• Blocks damaging ultraviolet (UV) light as the sleeve that covers 94% of the bottle surface.
• Protects the glass surface from damage.
• Facilitates safer handling as the silicone provides a much better grip than glass. Glass can become very slippery especially if the bottle has been sprayed with 70% alcohol for surface disinfection.
• Unlike amber bottles or aluminium foil, the Light Shield has two viewing windows that allow easy checking of the phenol red indicator colour, or for signs of microbial contamination.

3. How chemically resistant is DURAN® TILT Protective Light Shield?
The silicone rubber used for the Light Shield has an excellent resistance to solvents and other chemicals. It is virtually unaffected by polar organic solvents, or dilute acids or dilute alkalis. The silicone rubber will swell and soften in non-polar organic solvents, but does not dissolve or decompose. Strong acids and alkalis will attack silicone rubber and should be avoided.
4.What is the heat resistance of the DURAN® TILT Light Shield?
The recommended usage temperature range for the Light Shield is -30 °C to +200 °C. At temperatures below -30 °C the material will become brittle, and above about +220 °C the material will begin to decompose.
5. What is the recommended method of cleaning the DURAN® TILT Light Shield?
We recommend that the Light Shield is removed from the TILT bottle before cleaning.
The Light Shield may be cleaned either by hand, or in an automatic washing process using neutral (around pH 7.0) detergents. Do not use cleaners containing solvents, or chlorine.
For further information about cleaning labware, download our free guide (available in English, German or French) at:
www.aklab.de
6. Will the white DURAN® TILT Light Shield be available in other colours?
Maybe in the future, let us know what colours you think we should offer.

d) DURAN® GL 56 Bottle Tag FAQs

1. What could the coloured GL 56 Bottle Tags be used for?

The GL 56 Bottle Tags are available in a range of four colours (orange, yellow, blue, and purple) and are a temporary way to easily colour code / tag the TILT Bottles They offer a semi-permanent solution to the marking of bottles and avoid the damage caused by “scratch marking” the glass surface. The silicone is chemically robust, heat resistant, fully autoclavable, machine washable and re-usable.
There are many potential uses such:
• If there is a central wash facility, you could tag the TILT bottles to ensure that you get your own bottles returned!
• Use specific colours to identify media used for particular cell lines.
• Identify all the bottles used for one batch of media with one colour.
• Identify different batches of media produced in a central facility.

2. What material are the coloured GL 56 Bottle Tags made from?
The GL 56 Bottle Tags are moulded from a food grade silicone rubber.
3. Will the GL 56 Bottle Tags be available in additional colours?
Maybe in the future, let us know what colours you think we should offer.
4. How chemically resistant are the coloured GL 56 Bottle Tags?
The silicone rubber used for the GL 56 Bottle Tags has an excellent resistance to solvents and other chemicals. It is virtually unaffected by polar organic solvents, or dilute acids or dilute alkalis. The silicone rubber will swell and soften in non-polar organic solvents, but does not dissolve or decompose. Strong acids and alkalis will attack silicone rubber and should be avoided.
5. What is the heat resistance of the coloured GL 56 Bottle Tags?
The recommended usage temperature range for the GL 56 Bottle Tags is -30 °C to +200 °C. At temperatures below -30 °C the material will become brittle, and above about +220 °C the material will begin to decompose.
6. What is the recommended method of cleaning the GL 56 Bottle Tags?
The Bottle Tags may be cleaned either by hand or in an automatic washing process using neutral (around pH 7.0) detergents. Do not use cleaners containing solvents, or chlorine. For further information about cleaning labware, download our free guide (available in English, German or French) at: www.aklab.de

e) TILT GL 56 Screw Cap Label FAQs

1. What material are the TILT self-adhesive labels made from?
The TILT self-adhesive labels are made from writable / printable white polyester with a high strength adhesive optimised for adhesion to polypropylene.
2. How robust are the TILT self-adhesive labels?
The TILT self-adhesive labels are designed to be stay on during autoclaving processes, storage in fridges and freezers, and submersion in water baths.
3. What is the heat resistance of the TILT self-adhesive labels?
The TILT self-adhesive labels can be used over a wide temperature range from - 40 to 150 °C. For the best bond, the TILT bottles and caps should be labelled at room temperature or higher. The minimum temperature that the labels should be applied is +5 ºC. Lower temperature surfaces will prevent the adhesive for developing the maximum contact with the substrate. We recommend that to achieve the maximum bond the labels are rubbed down using finger pressure.
4. What pens and markers do you recommend to use with the TILT self-adhesive labels?
We have tested a wide range of standard laboratory markers and found that black general laboratory markers worked best with the labels. The labels can also be printed in ink jet or laser printers.
5. Are the TILT GL 56 screw cap labels printable?
The labels are supplied in a printer friendly format of six circular labels per 4” x 6" sheet. The label centres, and spacing is compatible with the Avery® print templates 41461 and 41465. (Templates are available for Microsoft® Word, Adobe® Illustrator®, Adobe® InDesign® and Adobe® Photoshop)*
*All product names, logos, and brands are property of their respective owners. All company, product and service names used in this FAQ document are for identification purposes only. Use of these names, logos, and brands does not imply endorsement.
6. How should the TILT self-adhesive labels be stored?
The TILT self-adhesive labels should be stored in dark and dry conditions at room temperature 21 ºC (+/- 10 ºC) and 50% (+/-15%) relative humidity.
7. How should the TILT self-adhesive labels be removed from the TILT GL 56 caps?
The combination of polyester and adhesive mean that the labels can be peeled off in one piece. However care must be taken to ensure the labels are peeled smoothly and slowly to avoid tearing the labels.
8. Sometimes there is a small amount of adhesive is left on the plastic after the TILT label has been peeled off. How should this be removed?
If the labels have been subjected to longer than usual autoclave cycles or high temperature heating cycles, the adhesive can sometimes remain on the plastic. This residue can be easily removed using acetone, isopropanol or ethanol.
9. What is the chemical resistance of the TILT self-adhesive labels?
The labels are resistant to limited expose to the following chemicals (as tested under defined conditions*):
• Deionized water
• 1X Phosphate buffered saline
• 10% Bleach solution (1:10 v/v)
• 70% Ethanol
• 70% Isopropyl alcohol
• 10% Sodium hydroxide solution
• 10% Sulphuric acid solution
• 10% Hydrogen peroxide solution

*TILT Labels marked by pen were laminated to DURAN® screw caps. Tests were performed at room temperature. Testing consisted of 5 cycles of 10 minute immersions in the specified test fluid followed by a 30 minute recovery period. After final immersion, samples rubbed 10 times with cotton swab saturated with test fluid.
10. Are the TILT labels hazardous in any way?
No. The labels are non-hazardous, and free of toxic heavy metals. The printed labels are safe to dispose in general waste stream.

f) DURAN® Bottle Top Filtration Units FAQs

1. What level of vacuum is recommended for optimal performance of the DURAN® Bottle Top filter units?

Recommended vacuum:
- 507 mbar / 50.7 kPa / 15 inch of mercury

Maximum Operating Vacuum:
- 677 mbar / 67.7 kPa / 20 inch of mercury

2. Why are there three pore sizes available?
The pore sizes are for the three types of filtration typically used in cell culture media preparation:
0.1μm   Mycoplasma removal
0.2μm   Filter sterilization and ultracleaning of aqueous solutions
0.45μm Clarification of aqueous solutions
3. Which membrane filter pore size should I use for sterilization?
We recommend the use of a 0.2 μm pore size membrane filter as the final filter for any sterilizing filtration. The 0.2 μm membranes used in the DURAN bottle top filters have been tested and shown to retain 100% of the Brevundimonas diminuta challenge bacteria at a concentration of 1x 10-7 cfu per cm2 using ASTM methodology.
4. Which materials are the DURAN® Bottle Top Filtration Units manufactured from?
The DURAN Filtration units are manufactured from USP <87> Class VI Materials, the funnel body is moulded from polystyrene and contains a polyethersulfone (PES) membrane. The membrane is surfactant free, and has low protein binding and extractables.
5. What is the thread on the DURAN® Bottle Top Filtration Units?
The DURAN Filtration units are offered with the industry standard 45 mm thread compatible with GL 45 media bottles. The re-usable GL 45 thread adaptor must be used to make the filter units compatible with the GL 56 thread of the TILT bottle.